Methods of Genome Editing Oocytes

Abstract

Researchers at the University of California, Davis have developed a way to introduce large genetic modifications in livestock species, in a high throughput manner.

Full Description

This platform technology reduces the overall steps and complexity of introducing genetic changes via microinjection into livestock, and instead utilizes a combination of techniques and chemically modified oocytes to produce up to 100 gene-edited zygotes in parallel; donor DNA templates of up to 4.7 kb are possible. This technology helps overcome challenges such as long generation intervals and linkage drag associated with traditional breeding approaches.

Applications

• Introduction of beneficial traits in livestock species 
• Elimination of detrimental traits in livestock species

Features/Benefits

• Allows precise genetic alterations into livestock genomes 
• Scalable and high-throughput production of gene-edited animals 
• Eliminates the need for specialized equipment and personnel 
• Bypasses slower traditional breeding processes 
• Larger (up to 4.7kb) donor DNA templates possible

Patent Status

Additional Patent Pending