Engineering Cas12a Genome Editors with Minimized Trans-Activity
Tech ID: 32640 / UC Case 2022-071-0
The inventors engineered a set of LbCas12a mutants through rational design and directed evolution. The engineered mutants can function as efficient genome editors with minimized trans-activity.
Suggested uses for the engineered LbCas12a mutants with minimized trans-activity include:
- reduced off-target genome editing in eukaryote cells.
- development into other molecular tools for genome editing.