CRISPR-CAS EFFECTOR POLYPEPTIDES AND METHODS OF USE THEREOF (“Cas-VariPhi”)

Tech ID: 32134 / UC Case 2021-027-0

Patent Status

Patent Pending

Brief Description


CRISPR-Cas systems include Cas proteins, which are involved in acquisition, targeting and

cleavage of foreign DNA or RNA, and a guide RNA(s), which includes a segment that binds Cas proteins and a segment that binds to a target nucleic acid. For example, Class 2 CRISPR-Cas systems comprise a single Cas protein bound to a guide RNA, where the Cas protein binds to and cleaves a targeted nucleic acid. The programmable nature of these systems has facilitated their use as a versatile technology for use in modification of target nucleic acid.

 

UC Berkeley researchers have discovered a novel family of proteins (CasVariPhi) that utilize a guide RNA to perform RNA-directed cleavage of nucleic acids. Viral and microbial (cellular) genomes were assembled from a variety of environmental and animal microbiome sources, and variants of a novel and previously unknown Cas protein family were uncovered from the sequences decoded. 

Suggested uses


 

  • gene editing of bacterial, archaeal, and eukaryotic cells
  • transcription repression of specific genes using inactivated CasVariPhi
  • targeting of proteins bound to CasVariPhi to a specific locus of a genome
  • diagnostic applications via trans-cleavage activity

 

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Inventors

  • Doudna, Jennifer A.

Other Information

Keywords

CRISPR, CasVariPhi, gene editing

Categorized As

Additional Technologies by these Inventors