Novel Phage CRISPR-Cas Effectors and Uses Thereof
Tech ID: 31724 / UC Case 2020-066-0
UC Berkeley researchers have discovered a novel family of proteins denoted Cas12L within the Type V CRISPR Cas superfamily distantly related to CasX, CasY and other published type V sequences. These Cas12L proteins utilize a guide RNA to perform RNA-directed cleavage of DNA.
- Targeted genome editing of bacterial, archaeal, and eukaryotic cells
- Transcription repression of specific genes using inactivated Cas12L
- Targeting of proteins bound to Cas12L to a specific locus of a genome
- Diagnostic applications via trans-cleavage activity