Improved Plant Regeneration Method Using GRFs, GIFs or Chimeric GRF-GIF Proteins
Abstract
Researchers at the University of California, Davis and the Institute of Molecular and Cellular Biology of Rosario in Argentina have collaborated to develop methods for improving plant regeneration efficiency using transformations via a GRF, a GIF, or a GRF-GIF chimera.
Full Description
Gene editing has revolutionized the development of new plant varieties. Unfortunately, many important crops species exhibit prohibitively low regeneration efficiencies and are therefore incompatible or minimally compatible with gene editing. There is, therefore, a significant need to improve plant regeneration.
Researchers
have collaborated on methods for improving plant regeneration efficiency that
apply various Growth-Regulating Factors (GRF), GRF-Interacting Factors (GIF) or
GRF-GIF chimera to transformed plant cells. These methods dramatically increase
regeneration efficiency, reduce the time required to regenerate transgenic
plants and can eliminate the need for plant selectable marker genes.
Applications
- Greatly increases regeneration efficiency in several plant varieties
- Accelerates the production of transgenic plants from leaf explants instead of embryos
- Increases the range of genotypes capable of being transformed efficiently
Features/Benefits
- Increases regeneration frequency of various crops with previously low transformation capacities
- Eliminates exposure to exogenous cytokinins required by many plant transformation systems
Patent Status
| Country | Type | Number | Dated | Case |
| United States Of America | Published Application | 20230032487 | 02/02/2023 | 2019-562 |
Related Materials
- A rapid and highly efficient sorghum transformation strategy using GRF4-GIF1/ternary vector system - 07/10/2024
- Use of GRF-GIF chimeras and a ternary vector system to improve maize (Zea mays L.) transformation frequency - 07/10/2024
- Overexpression of GRF-GIF genes enhances plant regeneration in cassava (Manihot esculenta) - 07/10/2024
Contact
- Eugene Sisman
- esisman@ucdavis.edu
- tel: View Phone Number.
Inventors
- Debernardi, Juan M
- Dubcovsky, Jorge
- Tricoli, David M.
Other Information
Keywords
Transformation, regeneration efficiency, GRF, GIF, wheat, crops; chimera, plant proteins
