Polyketide synthases are multifunctional enzymes that catalyze the biosynthesis of polyketides. These enzymes make attractive targets because they can be modified to produce commodity chemicals. The invention herein describes methods for producing polyketide synthase variants whose activity and/or substrate specificity can be tailored. An example of a polyketide synthase variant would be 2-pyrone synthase, which produces 2-pyrone. Other modified synthase variants can be generated to produce other key materials including ketides, lactones, etc.
Polyketides are a class of compounds that include antibiotics, immune-suppressants and anticancer agents. Polyketide synthase is a naturally occurring enzyme or engineered enzyme which can be used to prepare commercially useful chemical compounds. Applications of the polyketide synthase variant is applicable in the production of food additives, plasticizers, phytochemicals and phytochemical mixes, etc. Variants of polyketide synthases can have altered capabilities that could expand the enzymatic toolbox for enzyme-based chemical synthesis, and can demonstrate increased enzymatic activity.
Efficient methods for production of polyketide synthase variants are needed. he inventors have developed the following:
· Efficient methods for production of polyketide synthase variants;
· Polyketide synthase variants that have increased activity, stability and solubility;
|United States Of America||Published Application||20170073649||03/16/2017||2017-581|
|Patent Cooperation Treaty||Published Application||2015134807A1||09/11/2015||2017-581|