Superresolution Microscopy And Ultrahigh-Throughput Spectroscopy

Tech ID: 25210 / UC Case 2016-008-0

Patent Status

Country Type Number Dated Case
United States Of America Issued Patent 10,151,701 12/11/2018 2016-008
Patent Cooperation Treaty Published Application WO2017027818 02/16/2017 2016-008

Brief Description

Current super-resolution microscopy (SRM) methods have excellent spatial resolution, but no spectral information. Issues such as heavy color crosstalk, compromised image quality, and difficulties in aligning 3D coordinates of different color channels mean that high-quality multicolor 3D SRM remains a challenge. Another current imaging technique, single-molecule spectroscopy, is also limited in use because current methods are low throughput, have low spatial resolution, and cannot be used effectively for densely labeled biological samples.


UC Berkeley researchers have developed a 3-D super-resolution microscopy and single molecule spectroscopy system that addresses the issues inherent to both of these imaging techniques. By synchronously measuring the fluorescence spectra and positions of millions of single molecules within minutes, both spectrally resolved SRM and ultrahigh-throughput single-molecule spectroscopy are made possible.

Suggested uses

  • Multicolor, 3D super-resolution microscopy
  • Ultrahigh-throughput single-molecule spectroscopy



  • Works as stand-alone system or module added onto an existing super-resolution microscopy system
  • No crosstalk between different color channels
  • 3D images of different color channels automatically aligned
  • High throughput


Learn About UC TechAlerts - Save Searches and receive new technology matches


  • Xu, Ke

Other Information


Super-resolution, microscopy, spectroscopy, 3D, multicolor, high throughput, imaging

Categorized As