|United States Of America||Issued Patent||10,151,701||12/11/2018||2016-008|
|Patent Cooperation Treaty||Published Application||WO2017027818||02/16/2017||2016-008|
Current super-resolution microscopy (SRM) methods have excellent spatial resolution, but no spectral information. Issues such as heavy color crosstalk, compromised image quality, and difficulties in aligning 3D coordinates of different color channels mean that high-quality multicolor 3D SRM remains a challenge. Another current imaging technique, single-molecule spectroscopy, is also limited in use because current methods are low throughput, have low spatial resolution, and cannot be used effectively for densely labeled biological samples.
UC Berkeley researchers have developed a 3-D super-resolution microscopy and single molecule spectroscopy system that addresses the issues inherent to both of these imaging techniques. By synchronously measuring the fluorescence spectra and positions of millions of single molecules within minutes, both spectrally resolved SRM and ultrahigh-throughput single-molecule spectroscopy are made possible.