A new strategy for barcoding single living cells using lipid-modified oligonucleotides that can vastly enhance sample multiplexing in droplet microfluidics-based RNA sequencing
Single-cell RNA sequencing has recently emerged as a powerful tool for mapping transcriptional changes in heterogeneous cell populations. Recently, large-scale genomic screens combined with single-cell RNA sequencing have been utilized to understand complex biological phenomena. Novel insights could also be gained from coupling single-cell RNA sequencing to chemical library or drug screens, but methods for stably labeling living cells with oligonucleotide barcodes are lacking. Lipid-modified oligonucleotides represent an inexpensive, scalable, and technically simple method for labeling cell membranes in a fashion that interfaces with existing single-cell RNA sequencing workflows using droplet microfluidics.
This new cell barcoding method provides the following advantages:
Researchers at University of California, San Francisco have developed a new cell barcoding method that uses lipid-conjugated oligonucleotides to efficiently label single live cells derived from distinct patients or test conditions. Oligonucleotide barcodes (engineered with a PCR handle, unique identifier and PolyA sequence) can be subsequently introduced to the cells and subsets of the cells processed for droplet microfluidics-based RNA sequencing library preparation. This method can be commercially applied in the form of 96-, 384-, 1536- or 3456-well plates containing lipid-modified oligonucleotides prehybridized to sample barcodes. Cells derived from distinct perturbations or clinical samples could be barcoded via dispensing into unique wells upstream of labeling and single-cell RNA sequencing.
To develop and commercialize this technology, potentially as a cell barcoding kit for droplet microfluidics-based RNA sequencing.
Single cell RNA sequencing library preparation
Proof of Concept
|European Patent Office||Published Application||3818151||05/12/2021||2018-119|
Additional Patents Pending
Single Cell RNA Sequencing, Barcoding, Droplet microfluidics, Library preparation, Clinical & preclinical samples