Novel Diagnostic Methods for Citrus Stubborn Disease

Tech ID: 24526 / UC Case 2012-622-0

Patent Status

Country Type Number Dated Case
United States Of America Issued Patent 9,389,230 07/12/2016 2012-622
United States Of America Published Application 20190195873 06/27/2019 2012-622



Brief Description


Citrus greening disease, also known as huanglongbing (HLB), is a stubborn bacterial disease caused by insect-transmission and phloem-limited bacterial pathogens. It has been a serious threat to the US citrus industry, decimating many citrus trees and costing the economy $11B in damages annually. The most commonly used method of nucleic-acid based pathogen detection has issues with low-titer (low concentration of antibodies to antigen) and cannot handle the erratic distribution of the pathogens.


Brief Description:

UCR researchers have developed a proof-of-concept for using secreted proteins of bacterial pathogens to detect bacterial diseases. These abundant and stable secreted proteins serve as robust detection markers for immunoassay-based diagnostics. Compared to current methods, this novel method is more high-throughput, economical, and able to monitor the pathogens dispersed throughout the plant transportation system.


  • Robust detection - secreted proteins are abundant and stable
  • Rapid, cost-effective processing of large samples
  • Monitors variable distribution of pathogens, and not just at the site of infection
  • Proof-of-concept experiment available in a simple kit


  • Infected trees where pathogens are unevenly distributed
  • Field survey for plants


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Other Information


citrus, citrus pathogens, citrus stubborn disease, CSD, HLB, S. Citri, fresh fruit, global foods, antibody-based serological detection, immunoassay-based diagnostic, plant bacterial disease, plant bacterial pathogen, huanglongbing, HLB, citrus greening disease, infected trees, tree disease, insect-transmitted disease, phloem

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