|United States Of America||Issued Patent||10,228,554||03/12/2019||2014-118|
|Patent Cooperation Treaty||Published Application||WO2015134924||09/11/2015||2014-118|
Phase imaging has applications in biology and surface metrology, since objects of interest often do not absorb light but cause measurable phase delays (e.g. biological cells or uneven surface heights). Here, a new extension to an experimentally simple method for imaging quantitative phase information is described, which uses a Kalman filter algorithm with a stack of intensity images taken through focus. The extended method involves incorporation of information about the microscope source shape in Koeler configuration, so that the coherence of the illumination may be included into the phase retrieval algorithm in order to produce more accurate phase results with arbitrary source shapes and sizes.
Investigators have optimized and extended the Kalman filtering method to reduce computational complexity and to produce images using partially coherent illumination. This new software and method is faster and more efficient than previous methods, and in addition is robust to noise. It is compatible with a range of imaging systems, including optical, electron, X-ray and synchrotron, for example. Further, modifications are described for variations on the phase contrast mechanism, such that any complex transfer function (including but not limited to defocus) may be used.
Imaging systems, Phase contrast microscopy, Quantitative phase imaging, Kalman filtering, Partially coherent illumination