Improved Saccharification Efficiency by Inhibiting a Xylosyltransferase

Tech ID: 23951 / UC Case 2012-162-0

Full Description

Pamela Ronald and researchers at the Joint BioEnergy Institute (JBEI) have identified a glycosyltransferase encoded by a rice gene that is critical for xylose biosynthesis in plant cell walls. Inhibiting the expression of the gene, Os02g22380, in plants reduces the plants’ lignin content, thus reducing recalcitrance of their cell walls and increasing the amount of soluble sugar that can be extracted from them. The technology is applicable to wheat, rice, corn, switchgrass, sorghum, millet, miscanthus, sugarcane, barley, turfgrass, hemp, bamboo and Bracypodium.

Mutant rice plants based on this finding demonstrated reduced height with leaves deficient in xylose, as well as ferulic acid and coumaric acid – acids linked with the inhibition of microbes’ ability to covert sugars to fuels. In addition, using a promoter to limit the action of this gene to non-vascular tissue improves plant height. For more details, see the publication linked below.

The Joint BioEnergy Institute (JBEI, is a scientific partnership led by the Lawrence Berkeley National Laboratory and including the Sandia National Laboratories, the University of California campuses of Berkeley and Davis, the Carnegie Institution for Science and the Lawrence Livermore National Laboratory. JBEI’s primary scientific mission is to advance the development of the next generation of biofuels.


  • Biofuel production


  • Pathway to more efficient saccharification in bioenergy plants
  • Increases soluble sugar extraction compared to wild type plants

Patent Status

Country Type Number Dated Case
United States Of America Issued Patent 10,233,457 03/19/2019 2012-162

State Of Development

The technology has been tested in rice plants, which demonstrated leaves deficient in xylose, ferulic acid and coumaric acid. Specifically, cell wall extracts given a hot water pre-treatment exhibit a 62% increase in the total sugars released in an enzyme mixture that contains cellulase, α-mannosidase, and hemicellulases. Phenolic composition indicates a strong decrease in ferulic acid (59%) along with a decrease in coumaric acid (44%), which are downstream inhibitors. For more details, see the publication linked below.

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  • Chiniquy, Dawn
  • Ronald, Pamela C.
  • Scheller, Henrik

Other Information


xylose biosynthesis, reduced lignin, improved saccharification

Categorized As