This technology identifies an advanced imaging technique of biomicroscopy using an innovative type of a wide-field multi-focus microscope to enable fast, high-resolution 3D imaging.
This technology provides the following advantages:
Today, many three-dimensional (3D) biological specimens and processes are studied in two-dimensions (2D) due to the slow speed of the light microscope in recording a 3D image. This technology advances imaging techniques of biomicroscopy using an innovative type of a wide-field Multi-Focus microscope to enable fast, high-resolution 3D imaging. The system includes a novel grating design and chromatic correction scheme that is appended to the camera port of a high-resolution epifluorescence microscope to produce an instant focal stack of high-resolution 2D images simultaneously displayed on a single camera. The 3D microscope is designed to minimize photobleaching and phototoxicity while enabling high-speed imaging of weak fluorescent samples such as single fluorophores and to acquire multiple focal planes without aberrations to avoid loss in resolution and contrast.
The concept of Multi-Focus imaging uses optical manipulation to produce an entire focal series instantaneously. The Multi-Focus system developed is based on a phase-only diffractive grating placed in the Fourier plane in the emission path. The grating pattern is specially designed to split up the emission light from the sample into a set of simultaneous images and to focus shift diffraction orders. Each diffraction order forms a separate image of the sample, and the resulting series of images is recorded as a 2D array on a single, large-chip electron multiplying charge couple device camera.
|United States Of America||Issued Patent||9,477,091||10/25/2016||2012-220|
|European Patent Office||Published Application||2802861||11/19/2014||2012-220|
Additional Patent Pending