One of the most common causes of loss of vision is by corneal endothelial dystrophy (CED). Moreover, Fuchs CED is the leading cause of age-related blindness in individuals over the age of 40 in the United States affecting ~ 4% of the population. The current standard of care is to perform restorative corneal transplantation, but due to a shortage of healthy human donors, this is a challenge confronting the medical community. One solution would be to develop alternative sources of transplantation material. Human corneal endothelial cells (CESs) are not proliferative and do not regenerate in vivo. Therefore, there is a major interest in development of in vitro expandable cell sources for engineering corneal endothelium.