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Type III CRISPR-Cas System for Robust RNA Knockdown and Imaging in Eukaryotes

Type III CRISPR-Cas systems recognize and degrade RNA molecules using an RNA-guided mechanism that occurs widely in microbes for adaptive immunity against viruses. The inventors have demonstrated that this multi-protein system can be leveraged for programmable RNA knockdown of both nuclear and cytoplasmic transcripts in mammalian cells. Using single-vector delivery of the S. thermophilus Csm complex, RNA knockdown was achieved with high efficiency (90-99%) and minimal off-targets, outperforming existing technologies of shRNA- and Cas13-mediated knockdown. Furthermore, unlike Cas13, Csm is devoid of trans-cleavage activity and thus does not induce non-specific transcriptome-wide degradation and cytotoxicity. Catalytically inactivated Csm can also be used for programmable RNA-binding, which the inventors exploit for live-cell RNA imaging. This work demonstrates the feasibility and efficacy of multi-subunit CRISPR-Cas effector complexes as RNA-targeting tools in eukaryotes.

RETROTRANSPOSON-BASED DELIVERY VEHICLE AND METHODS OF USE THEREOF

Gene therapy delivery generally falls into two main categories: viral-mediated and non-viral mediated delivery. Viral-mediated integrative approaches are most commonly used in dividing cells, where delivery is mediated, e.g., through the use of lentiviruses and retroviruses engineered to carry therapeutic DNA into cells. Such viruses have a number of drawbacks, e.g., disruption of the cell's function, the cell’s own machinery may silence expression and payload size limitations. Whereas class II transposons such as piggyBac, Sleeping Beauty, and Tol2 can integrate larger payloads; however, such transposons have a tendency to integrate in areas where active transcription is occurring. Therefore, there is a need in the art for delivery vehicles that provide for delivery of larger coding regions.    UC Berkeley researchers have developed a gene delivery system that has a nucleotide sequence encoding an R2 retrotransposon R2 polypeptide that is able to deliver one or more gene products of interest to a eukaryotic cell.

Flexible Piezoelectret Actuator/Sensor Patch For Mechanical Human-Machine Interfaces

Flexible and wearable devices with the capabilities of both detecting and generating mechanical stimulations are critical for applications in human-machine interfaces, such as augmented reality (AR) and virtual reality (VR). UC Berkeley researchers have developed a flexible patch based on a sandwiched piezoelectret structure is demonstrated to have a high equivalent piezoelectric coefficient of d33 at 4050 pC/N to selectively perform either the actuating or sensing function. As an actuator, mechanical vibrations with peak output force of more than 20 mN have been produced, similar to those from the vibration mode of a modern cell phone and can be easily sensed by human skin. As a sensor, both pressure detection limit of 1.84 Pa for sensing resolution and excellent stability of less than 1% variations in 6000 cycles have been achieved. The design principle together with the sensing and driving characteristics can be further developed and extended to other soft matters and flexible devices.

Ambient infrared laser ablation mass spectrometry (AIRLAB-MS) with plume capture by continuous flow solvent probe

UC Berkeley researchers and others developed a new experimental setup for spatially resolved ambient infrared laser ablation mass spectrometry (AIRLAB-MS) that uses an infrared microscope with an infinity-corrected reflective objective and a continuous flow solvent probe coupled to a Fourier transform ion cyclotron resonance mass spectrometer is described. 

Precision Graphene Nanoribbon Wires for Molecular Electronics Sensing and Switch

The inventors have developed a highly scalable multiplexed approach to increase the density of graphene nanoribbon- (GNR) based transistors. The technology forms a single device/chip (scale to 16,000 to >1,000,000 parallel transistors) on a single integrated circuit for single molecule biomolecular sensing, electrical switching, magnetic switching, and logic operations. This work relates to the synthesis and the manufacture of molecular electronic devices, more particularly sensors, switches, and complimentary metal-oxide semiconductor (CMOS) chip-based integrated circuits.Bottom-up synthesized graphene nanoribbons (GNRs) have emerged as one of the most promising materials for post-silicon integrated circuit architectures and have already demonstrated the ability to overcome many of the challenges encountered by devices based on carbon nanotubes or photolithographically patterned graphene. The new field of synthetic electronics borne out of GNRs electronic devices could enable the next generation of electronic circuits and sensors.  

ISOCHORIC IMPREGNATION OF SOLID FOODS AT SUBFREEZING TEMPERATURES

UC researchers and others have created a system and method for impregnating a targeted solid food item with impregnation fluids during the isochoric freezing process. The impregnation fluids are selected to enhance the quality of the targeted food item. Specifically, the isochoric freezing process is used to impregnate a fruit or vegetable with an impregnation solution. The solution is infused into the void pores of fruits and vegetables, without destroying cellular tissue. 

BALB STING Knockout Mice

These are mice on the BALB/cAnNCrl background that lack functional STING protein. A 5 bp deletion (GCCTC; nucleotides 402-406 of NM_001289591.1) was introduced to exon 3 of the mouse Sting1 gene via CRISPR/Cas9 methodologies