Salt-Tolerant Dna Polymerases
Tech ID: 32798 / UC Case 2011-760-0
Background
Various scientific and industrial applications exist in which it would be advantageous to use a DNA polymerase that function efficiently at high salt concentrations. In sequencing, GC compressions can be resolved by using high salt concentrations. In nanopore sequencing high salt concentration boosts the signal to noise ratio for ionic-current based nanopore measurements.
Technology Description
Methods of performing DNA synthesis using salt tolerant versions of DNA polymerases derived from Bacillus phage phi29 are described. Such polymerases can function in salt solutions with concentrations as high as 25% wt/volume of salt (KCl, NaCl or other monovalent salts).
Applications
Nucleic acid sequencing
Nucleic acid library generation
Intellectual Property Information
| Country | Type | Number | Dated | Case |
| United States Of America | Issued Patent | 10,059,984 | 08/28/2018 | 2011-760 |
| Patent Cooperation Treaty | Published Application | WO 2012/173905 | 12/20/2012 | 2011-760 |
Contact
- Jeff M. Jackson
- jjackso6@ucsc.edu
- tel: View Phone Number.
Inventors
- Bernick, David L.
- Holmes, Andrew
- Nivala, Jeffrey M.
Other Information
Keywords
Phi29, Halophilic, DNA polymerase, DNA libraries, Nucleic acid sequencing
