Researchers at the University of California, Davis have developed a method to produce haploid progeny plants from transgenic and wild-type plants that only carry chromosomes from the wild-type gamete.
True-breeding plant lines, or plants that produce genetically identical progeny, are required for the development and production of specific crop varieties. Conventional approaches to creating such lines involve inbreeding, taking 7-9 generations to achieve homozygosity. Haploid plants, or plants that carry the genome of a single (haploid) gamete can reduce the time by an order of magnitude to create true-breeding lines but are difficult to induce.
Researchers at the University of California, Davis have developed a method that utilizes Centromeric Histone H3 (CENH3) and small in-frame deletions in CENH3 to create true-breeding varieties. Using CRISPR/cas, this technology can produce healthy and fertile haploid progeny. Lines edited by this method from crossed CRISPR/cas and wild-type plants produce wild-type haploid progeny with little modification and are considered nontransgenic in many countries including the U.S. This technology provides a rapid and low-cost method to produce haploid-inducing plants and applicable to all plants susceptible to CRISPR mutagenesis.
Rights are available within certain fields. Further information is available upon inquiry.
Haploid Progeny, Botany, Plant Biology, CEH3, CRISPR, In-frame Deletions