CRISPR-based Graphene Biosensor for Digital Detection of DNA Mutations

Patent Status

Additional Patent Pending

Brief Description

UC Berkeley and Keck Institute researchers have reported the development and testing of a graphene-based field-effect transistor that uses CRISPR technology to enable the digital detection of a target sequence within intact genomic material. Termed CRISPR–Chip, the biosensor uses the gene-targeting capacity of catalytically deactivated Cas9 complexed with a specific single-guide RNA and immobilized on the transistor to yield a label-free nucleic-acid-testing device whose output signal can be measured with a simple handheld reader.  

Suggested uses

• Diagnostics
• Research tools

Advantages

CRISPR–Chip generates, within 15 min, with a sensitivity of 1.7 fM and without the need for amplification, a significant enhancement in output signal relative to samples lacking the target sequence.

Related Materials

• Detection of unamplified target genes via CRISPR–Cas9 immobilized on a graphene field-effect transistor - 03/25/2019