CRISPR-based Graphene Biosensor for Digital Detection of DNA Mutations

Tech ID: 29311 / UC Case 2018-137-0

Patent Status

Patent Pending

Brief Description

UC Berkeley and Keck Institute researchers have reported the development and testing of a graphene-based field-effect transistor that uses CRISPR technology to enable the digital detection of a target sequence within intact genomic material. Termed CRISPR–Chip, the biosensor uses the gene-targeting capacity of catalytically deactivated Cas9 complexed with a specific single-guide RNA and immobilized on the transistor to yield a label-free nucleic-acid-testing device whose output signal can be measured with a simple handheld reader.  

Suggested uses


  • Diagnostics
  • Research tools

Advantages

CRISPR–Chip generates, within 15 min, with a sensitivity of 1.7 fM and without the need for amplification, a significant enhancement in output signal relative to samples lacking the target sequence.

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Inventors

  • Conboy, Irina M.

Other Information

Keywords

CRISPR chip, biosensor, graphene

Categorized As