UCLA researchers have developed a novel high throughput mechanically activated sorting (MACS) device.
Cells are soft, viscoelastic materials whose main structural components are proteins and membranes and whose mechanical phenotype can be significantly altered during pathological transformations. These transformations are mechanically measureable. A mechanically cell measuring device is mechanically activated sorting (MACS). MACS is a single-layer microfluidic device with integrated sensing electronics and valve actuation. MACS are based on established work in the fields of microfluidics and MicroElectrical-Mechanical Systems (MEMS). MEMS devices are capable of accurately measuring mechanical properties of individual cells but are challenging to scale up as each cell has to be placed at a probe tip or flat deformation surface manually. Microfluidics, on the contrary, allow cells to be separated and confined to a flow and placed in a given location consistently. Combining MEMS and microfluidics capabilities, a high-throughput mechanical sorting device was created.
UCLA researchers have developed a novel mechanically activated sorting (MACS) device. This is the first high throughput device to obtain a direct measure of individual cell mechanical properties. The MACS allows a large population of cells (on the order of magnitude of 10^8 cells) to be analyzed at a rate on the order of 10^3 cells per second based on mechanical properties of the cell.
Heterogeneous populations can now be mechanically sorted and correlated to particular pathologies, genetics, epigenetics, or proteomic structures. This invention is the first high –throughput mechanical sorting device. With detection rates nearly two order of magnitude times faster than the existing technology, MACS divide individual cells into subpopulations based on real-time stiffness measurements.
Laboratory measurements have been performed.
|United States Of America||Issued Patent||10,302,408||05/28/2019||2012-282|
|United States Of America||Issued Patent||9,423,234||08/23/2016||2012-282|