Non-Transgenic Haploid Plant Induction Lines

Tech ID: 25280 / UC Case 2014-584-0


Researchers at the University of California, Davis have identified non-transgenic mutant plants capable of generating haploid offspring.

Full Description

Generation of haploid plants greatly improves the efficiency and effectiveness of the breeding process, allowing the production of fertile true-breeding homozygous lines in a single generation. Identification and selection of diploid plants with a desirable combination of traits is accomplished by screening many generations of progeny and remains a time, labor, and capital intensive venture. Current technologies utilized for inducing haploidy, however, may have limited applicability, entail transgenic approaches, require special skills, or can be very time consuming. In light of global population growth and arable land constraints, an extraordinary need exists for the development of new technologies for the acceleration of plant breeding.

Researchers at the University of California, Davis have developed a technology that offers a non-transgenic method of haploid plant induction. The method can be easily and quickly implemented by breeders all over the world. It combines the speed of transgenic haploid induction with non-transgenic techniques and circumvents the stigma and regulatory hurdles associated with genetically modified haploid inducers.


This novel technology has multiple applications, including:

  • Non-transgenic induction of haploidy for breeding purposes
  • Creation of doubled-haploid elite lines
  • Haploid plants for genomics research (contain only one version of each gene)
  • Creation of cytoplasmic male sterile lines with a desired genotype (The method can transfer paternal chromosomes into maternal cytoplasm. Currently, generating a cytoplasmic male sterile line with a desirable genotype requires many generations of backcrossing, whereas this requires a single step.)


  • The time required to obtain true breeding lines is greatly diminished as this methodology bypasses the need for extensive backcrossing, instead producing homozygous doubled haploid progeny in a single generation
  • This non-transgenic approach greatly reduces the skill, cost, and labor required for haploid plant production, and overcomes accessibility and regulatory hurdles that make the process readily applicable in a much wider global market

Patent Status

Country Type Number Dated Case
United States Of America Published Application 20180116141 05/03/2018 2014-584


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  • Britt, Anne B.
  • Chan, Simon R.
  • Kuppu, Sundaram

Other Information


haploid, plant breeding, non-transgenic

Categorized As