Novel Multiplex Assay Detects Citrus Pathogens

Tech ID: 24385 / UC Case 2014-045-0

Patent Status

Country Type Number Dated Case
United States Of America Issued Patent 10,273,547 04/30/2019 2014-045


Photo from Agricultural Research Service

Photo by Mmacbeth

Brief Description


Citrus greening disease, also known as huanglongbing (HLB), has been a serious, pervasive problem caused by a multitude of plant pathogens. It has decimated many citrus trees, drastically decreasing orange production and costing the US economy an estimated $11B every year. Currently, there is no cure for HLB, so the citrus industry is in dire need for a cost-effective method of early HLB detection.


Brief Description:

UCR Researchers have developed a means to detect and identify multiple plant pathogens for disease diagnosis, including citrus greening disease. By developing a novel multiplex RNA assay, they discovered ten targets of nine citrus pathogens and a citrus control gene. In addition to the assays, target-specific probes were designed and implemented to improve the pathogen detection process. These assays were also coupled with high-throughput robotic extraction and purification procedures, optimized for citrus tissues. Furthermore, they also developed a 3-plex DNA assay system along with 3 targets for simultaneous detection, identification and quantification of plant pathogens.


  • Increases uniformity and cost effectiveness
  • User-friendly assay procedures
  • Simultaneous detection, identification and quantification for the ten targets


  • Detection of endemic and exotic plant pathogens
  • Plant health monitoring services
  • Citrus disease management


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Other Information


fresh fruit, global foods, citrus, citrus canker, citrus stubborn disease, pathogen detection, HLB, Citrus Variegated Chlorosis

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