|United States Of America||Issued Patent||10,273,547||04/30/2019||2014-045|
Photo from Agricultural Research Service
Photo by Mmacbeth
Citrus greening disease, also known as huanglongbing (HLB), has been a serious, pervasive problem caused by a multitude of plant pathogens. It has decimated many citrus trees, drastically decreasing orange production and costing the US economy an estimated $11B every year. Currently, there is no cure for HLB, so the citrus industry is in dire need for a cost-effective method of early HLB detection.
UCR Researchers have developed a means to detect and identify multiple plant pathogens for disease diagnosis, including citrus greening disease. By developing a novel multiplex RNA assay, they discovered ten targets of nine citrus pathogens and a citrus control gene. In addition to the assays, target-specific probes were designed and implemented to improve the pathogen detection process. These assays were also coupled with high-throughput robotic extraction and purification procedures, optimized for citrus tissues. Furthermore, they also developed a 3-plex DNA assay system along with 3 targets for simultaneous detection, identification and quantification of plant pathogens.