Chimeric TLR9/TLR4 Reporter Cell Line
Brief Description
This is a cell line that expresses a chimeric protein consisting of the extracellular domain of Toll-like receptor 9 (TLR9) fused to the transmembrane and cytosolic domains of TLR4 (hereafter referred to as TLR9N4C. TLRs are innate immune receptors that respond to microbial ligands and in some cases self-ligands. Responses to self- ligands can lead to autoimmune or autoinflammatory diseases.
Suggested uses
• Screening for compounds that impact activation of TLR9 (activators or inhibitors)
• Determination of mechanism of action of said compounds (chimeric receptor separates ligand binding from intracellular signaling.
• Detection of TLR9 ligands in samples
Advantages
The development of inhibitors of certain TLRs has been a goal of the pharmaceutical industry. A primary application of this TLR9N4C-expressing cell line is to screen for inhibitors of TLR9. UCB is willing to license this cell line to screen for inhibitors of TLR9 activation.
Related Materials
Contact
- Craig K. Kennedy
- craig.kennedy@berkeley.edu
- tel: View Phone Number.
Inventors
- Barton, Gregory M.
Other Information
Keywords
TLR9 Cell LIne
