The uniformity of each droplets volume in a digital microfluidic system is useful and often critical to the overall functions. Digitization of liquids, e.g. generating droplets from liquid reservoirs, is a key step to determine the droplet volume. Reasonable level of accuracy can be achieved by simple signal switching during the digitizing processes. However, the performance is subject to random variations over the devices and operation conditions. The known feedback mechanisms to control droplet uniformity required external equipments (pumps and valves) in the system hardware and closed the loop once per droplet in the feedback algorithm (i.e., the feedback is droplet-to-droplet).
UCLA researchers have developed an on-chip, real-time feedback system that accurately detects and controls the droplet volume. The system hardware uses no external pumps and valves, and the feedback algorithm employs continuous loop closing during each droplet generation (i.e., the feedback is within a droplet). With the developed feedback mechanism, the standard deviation of the generated droplet volumes was 5x smaller than that without feedback. Furthermore, the invented feedback allows generation of any droplet volume within a range, not limited by the size of driving pads on the chip anymore. The researchers have integrated the feedback control and microfluidic device on a portable 7 x 5 board, achieving a volume precision better than 1%.
The technology has been experimentally verified. A system has been built, tested and it has been performance characterized.
|United States Of America||Issued Patent||9,266,076||02/23/2016||2007-244|