Monoclonal Antibody To Human Dna Polymerase Epsilon Catalytic Subunit

Abstract

Human DNA polymerase e contains two apparent subunits of >200 and 55 kDa. Purified polymerase e was used to prepare mouse antiserum capable of recognizing different polymerase e subunits. A mouse monoclonal was identified that had specific recognition for the catalytic subunit of polymerase e (200 kDa).

Reference:
J Biol. Chem. (1995) v270, pp7799-7808.

Applications

Can be used to specifically recognize the catalytic subunit of polymerase e.

Advantages

Does not bind to polymerase a, b, or d.