Monoclonal Antibodies Specific For Triazine Herbicides Including Atrazine.

Abstract

Simazine and atrazine haptens with mercaptopropionic acid and aminohexanoic acid spacers were synthesized and conjugated to proteins via N-hydroxysuccinimide active esters. Mabs derived from immunized mice had I50 values of 3 to 4 ppb. The EIAs are compatible with simplified methods for triazine extraction and concentration from soil and water. The limit of detection for atrazine was 0.05 to 0.1 ppb.

Reference;
Karu et al. Monoclonal Immunoassay of Triazine Herbicides Development and Implementation 1991. ACS Symposium Series No. 451 Immunochemical Methods for Trace Chemical Analysis. pp59-77.