UC Berkeley researchers have developed a method for single-stranded nucleic acid extraction from complex biofluids with DNA-wrapped carbon nanotubes. Large viral single-stranded nucleic acids can be captured by corresponding DNA-wrapped carbon nanotubes and can be concentrated for subsequent polymerase chain reaction (PCR) amplification. This method can extract nucleic acids without complicated manufacturing and experimental processes, can generate higher extraction yields than a conventional commercial PCR kit, and fits into the current PCR workflow while requiring minimal chemical reagents.
This simple and accurate method uses less inexpensive materials and extracts higher yields of virus-specific RNA than conventional commercial polymerase chain reaction (PCR) amplification kits. Furthermore, by increasing the extraction yield of SARS-CoV-2-specific RNA in PCR through workflow modifications, this technology increases the sensitivity and detection threshold of the PCR technique as a diagnostic test.Existing nucleic acid extraction kits contain specialized materials such as magnetic beads or membrane filters, which are vulnerable to shortages during periods of extensive PCR demands like the SARS-CoV-2 pandemic.
carbon nanotube, SARS-CoV-2, virus diagnosis, nucleic acid purification, nucleic acid extraction