A Simple In Line Digital Holography System For Measuring 3D Cell Shape
Tech ID: 32222 / UC Case 2020-207-0
Technology Description
The invention disclosed is a simple in-line holographic method without the use of a separate reference beam for morphological analysis of cells. This technology will be especially useful in measuring three dimensional measurements such as thickness, shape, and volume of single cells (i.e. individual blood cells) or groups of cells. Previously, this approach required a separate reference beam for holographic imaging. However, the present technology achieves this objective without the separation of the reference beam and by increasing the distance between the cell and the hologram plane.
Value Proposition
- Cellular volumetric data can be calculated for live and fixed cells with real time imaging and analysis.
- Captured data is in the megabyte range, hence allowing the use of less intensive data storage and processing conditions.
- Faster workflow as multiple inline focal depth data are simultaneously obtained without the need to shift the light source or sample.
- Less than 10% measurement error.
Data Availability
Under CDA upon request
Patent Status
| Country | Type | Number | Dated | Case |
| Patent Cooperation Treaty | Published Application | WO 2022/032074 | 02/10/2022 | 2020-207 |
State Of Development
Proof of concept
Contact
- Catherine Smith
- Catherine.Smith2@ucsf.edu
- tel: View Phone Number.
Inventors
- Ohgami, Robert
Other Information
Keywords
microscopy, single cell analysis, holography, imaging, cell volume