UCLA researchers in the Department of Molecular, Cell, and Developmental Biology have discovered two Arabidopsis proteins, BIC1 and BIC2, that are capable of inhibiting light-dependent dimerization of cryptochrome (CRY) molecules. These BICs can be used as an improved drug screening platform through controlled, titratable, label-free and reversible protein – protein interactions.
Cryptochromes are blue-light receptors that are involved in photomorphogenesis in plants and are critical components of the human circadian clock, which is associated with numerous human diseases, including diabetes, obesity, cancer, mania etc. The Arabidopsis cryptochrome 2 (CRY2) undergoes blue light-dependent dimerization via disulfide bonds, resulting in activation of the photoreceptor necessary for light-dependent growth and reproduction. The light-dependent CRY2-CIB1 interaction has been utilized as an optogenetics tool to achieve light-induced regulation of transcription, protein translocation, DNA recombination, phosphoinositide metabolism, epigenetics change, and reversible protein inactivation trap in molecular mechanistic studies and drug discovery studies. The CRY2-CIB1 interaction also requires CRY2 dimerization. Therefore, a technology that can control CRY dimerization can be used as a regulator of CRY2-CIB1 interaction-dependent optogenetics tools, and most importantly, as a novel approach to regulate CRY and circadian clock activity in human cells in many disease models.
Researchers at UCLA have discovered two Arabidopsis proteins, blue-light inhibitors of CRY1 and CRY2 (BIC1, BIC2), that inhibit light-dependent formation of disulfide bonds between CRY molecules, leading to the suppression of CRY dimerization, CRY phosphorylation, and all physiological functions of cryptochromes. These Arabidopsis BICs not only function in plant cells- they also demonstrate activities in human cells.
- Regulators of any optogenetics method that relies on the light-dependent CRY2-CIB1 interaction
- Drug screening applications through titratable, label-free and reversible protein – protein interactions
- Regulators of CRY and circadian clock activity in human cells
|United States Of America||Published Application||20190300875||10/03/2019||2015-354|
cryptochrome, CRY, cryptochrome dimerization, light-dependent dimerization, optogenetics, circadian clock, transcription regulation, protein translocation, DNA recombination, phosphoinositide metabolism, epigenetics change, reversible protein inactivation