dCas9 Epigenome Editing Toolkit

Tech ID: 29108 / UC Case 2017-554-0

Abstract

Researchers at the University of California, Davis have developed a dCas9 toolkit for human epigenome editing.

Full Description

Epigenome editing is a rapidly developing field. Proteins are used to control the expression of specific genes in cells by controlling epigenetic information. Distinct histone epigenetic marks have been associated with gene expression, but information regarding the causal relationship is lacking.

Researchers at the University of California, Davis have developed a programmable dCas9 toolkit that allows for the precise control of the epigenome. The method uses fusions between proteins with methylation activity and dCas9 to alter epigenetic marks on histones (H3K27me3) at specific DNA sequences. These fusions can be used to test the effects of sequence-specific epigenetic marks on gene expression.

Applications

  • Epigenome editing

Features/Benefits

  • Specific sites in the human genome
  • Repressive histone modifications are not required for gene repression
  • Easily programmable
  • Precise control
  • Can be used to dissect the relationship between the epigenome and gene regulation

Patent Status

Patent Pending

Contact

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Inventors

  • O'Geen, Henriette
  • Segal, David J.

Other Information

Keywords

epigenetic editing, dCas9, H3K27me3, artificial transcription factor, histone methylation

Categorized As