Xanthene-Based Dyes For Voltage Imaging

Tech ID: 27603 / UC Case 2017-143-0

Patent Status

Patent Pending

Brief Description

Rapid changes in the membrane potential of excitable cells (e.g., neurons and cardiomyocytes) play a central role in defining the cellular signaling and physiological profiles of these specialized cells. Typically, the membrane potential is monitored and measured via patch clamp electrophysiology, which involves the use of a micro-electrode attached to or near the cell of interests.  Unfortunately, the use of an electrode is highly invasive, limits records to the soma of a single cell and is extremely low throughput.

Researchers at the University of California, Berkeley have designed and synthesized a voltage sensitive indicator that can provide excitation and emission profiles greater than 700 nm, and as such, represents an important method for visualizing membrane potential in living cells.

Suggested uses


  • Visualize membrane potential in living cells (especially good for neurons and cardiomyocytes)
  • Research tool
  • Drug Screening (e.g., drug interactions and complications with ion channels in the heart)

 

Advantages


  • Simple protocol
  • Unique excitation (the rate of electron transfer, fluorescence intensity, and quenching are altered in response to changes in transmembrane potential)

 

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Inventors

  • Miller, Evan W.

Other Information

Keywords

imaging, dye, neurons, cells, fluorescent

Categorized As