Transforming Growth Factor-Beta (TGF-β)-Responsive Single-Chain Variable Fragments And Chimeric Antigen Receptors

Tech ID: 27499 / UC Case 2016-076-0

Summary

UCLA researchers in the Department of Chemical and Biomolecular Engineering have developed chimeric antigen receptors that are responsive to human and mouse TGF-β (Transforming Growth Factor-Beta).

Background

TGF-β is a pleiotropic cytokine found at high levels in a variety of pathogenic states, including solid tumors, fibrosis, and dysregulated wounds. Many anti-TGF-β antibodies exist, but few anti-TGF-β scFvs have been described.scFvs and antibodies share the ability to bind tightly and specifically to target proteins, in this case TGF-β.However, scFvs are superior due to the following features: the smaller size of scFv facilitates smaller packaging and lower production costs, scFvs allow for more flexibility when designing applications, and scFvs are easier to genetically engineer.

Innovation

Researchers at UCLA have successfully described the sequences of scFvs that neutralize human and mouse TGF-β as well as chimeric antigen receptors (CARs) that robustly activate primary human T cells in response to TGF-β.

Applications

Therapeutics for diseases where high levels of TGF-β are present

Advantages

The TGF-CAR can be combined with tumor-targeting CARs or T cell receptors to render therapeutic T cells resistant to the immunosuppressive effects of TGF-b

Technical attributes of scFvs facilitate ease of production:

  • Apply less stress to gene delivery modalities
  • Can be collected directly from media in which it was produced
  • More easily used in genetic engineering and gene therapy applications

 

Related Materials

Contact

Inventors

  • Chen, Yvonne Y.

Other Information

Keywords

TGF-ß, Transforming growth factor-beta, chimeric antigen receptors, antibody, single-chain variable fragments, tumors, fibrosis, dysregulated wounds, therapy, immunotherapy, T-cell therapy, genetic engineering, gene therapy

Categorized As

UCLA Technology Development Group
10889 Wilshire Blvd., Suite 920,Los Angeles,CA 90095
tdg.ucla.edu
Tel: 310.794.0558 | Fax: 310.794.0638 | ncd@tdg.ucla.edu