Method for Making Electrophysiologically Mature Cardiomyocytes Using a Non-Genetic Approach

Tech ID: 27140 / UC Case 2014-580-0

Abstract

Researchers at the University of California, Davis have developed a novel method for inducing maturity in cardiomyocytes by altering cell phenotype by mimicking the pacing environment.

Full Description

Although heart transplants have become commonplace in medicine, the procedure does not create immaculate results. Using immature stem-cells has been suggested to be more ischemic-resistant and tolerate to a hyper toxic environment after transplantation. However, the immaturity of the cells can cause other issues such as an arrhythmogenic heart.

Researchers at the University of California Davis have developed a novel method using electrical pacing which induces a more mature, adult-like electrophysiology and excitation-contraction coupling in human pluripotent stem cell-derived cardiomyocytes. This is done by placing the cells in a culture using a field electric stimulator which generates electrical pluses delivered through two carbon electrodes to initiate action potentials and subsequent contractions in the cells.Stability of the cell membrane potential increased with electoral pacing that reduces the automaticity of stem-cell derived cardiomyocytes. This may improve the initiation of ectopic beats by these cells after transplantation.

Applications

  • Inducing maturity in pluripotent stem cell-derived cardiomyocytes
  • More accurate heart monitoring
  • Drug discovery
  • More accurate cardiotoxicity screening

Features/Benefits

  • May improve initiation of ectopic beats by the cells after transplantation
  • Does not genetically modify cells
  • Alters cell phenotype by mimicking cell environment

Patent Status

Country Type Number Dated Case
United States Of America Issued Patent 10,087,436 10/02/2018 2014-580
 

Additional Patent Pending

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Inventors

  • Li, Ronald
  • Lieu, Deborah K.

Other Information

Keywords

Cardiomyocytes, Electrophysiology, Human Pluripotent Stem Cell, Maturation

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