Fluorescent Biosensor for Cyclic GMP-AMP (cGAMP)

Tech ID: 25608 / UC Case 2016-086-0

Patent Status

Country Type Number Dated Case
United States Of America Published Application 20190161754 05/30/2019 2016-086
Patent Cooperation Treaty Published Application WO2017218358 12/21/2017 2016-086
 

Additional Patent Pending

Brief Description

The cGAS-cGAMP-STING pathway is an important immune surveillance pathway which gets activated in presence of cytoplasmic DNA either due to a microbial infection or a patho-physiological condition, including cancer and autoimmune disorders. Sensing 2’3’ cGAMP level is important in diagnostics perspective as well as in basic understanding of their regulation.  Small molecule activators of this pathway have also been shown to activate an anti-cancer immune response and thus an important use for pharmaceutical applications. However, a high throughput method to screen for such potential drugs is still not available.

UC Berkeley researchers have designed a RNA-based fluorescent biosensor for directly detecting 2’3’ cGAMP. The biosensor was able to detect 2’3’ cGAMP and assay cGAS activity in vitro and thus would be useful for high throughput screening of small molecule modulators of cGAS activity.  The biosensor was sensitive enough to quantify 2’3’ cGAMP in dsDNA- stimulated mammalian cell extracts. 

Suggested uses

 

  • In vitro assay of cGAS activity
  • High throughput screening platform for modulators of cGAS activity
  • Cancer immunotherapy    

 

Advantages


  • More direct method of detection without activating a conventionally used reporter 

 

Publication

An RNA-Based Fluorescent Biosensor for High-Throughput Analysis of the cGAS-cGAMP-STING Pathway An RNA-Based Fluorescent Biosensor for High-Throughput Analysis of the cGAS-cGAMP-STING Pathway 

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Inventors

  • Hammond, Ming Chen

Other Information

Keywords

Biosensor, cGAS, cGAMP, immunotherapy, infectious disease, autoimmune

Categorized As