Fluorescent Biosensor for Methyltransferase Assay

Tech ID: 25135 / UC Case 2015-196-0

Patent Status

Country Type Number Dated Case
United States Of America Published Application 20180267027 09/20/2018 2015-196
Patent Cooperation Treaty Published Application WO2017074955 05/04/2017 2015-196
 

Brief Description

Correct epigenetic regulation is essential to cellular development, and methyltransferases are enzymes important for epigenetic regulatory processes. They add methyl groups to their substrates, which can be DNA, proteins, or small-molecule secondary metabolites. Methyltransferases have been implicated in a number of diseases, including cancer, HIV infection, and diabetes, yet many remain uncharacterized.

S-adenosyl methionine (SAM) is used as a methyl group donor by a majority of methyltransferases. Use of SAM by a methyltransferase results in the production of S-adenosyl homocysteine (SAH). SAM is found across all branches of life, and therefore represents a useful biological marker for methyltransferase activity.

Researchers at UC Berkeley have developed a sensitive and selective means of assaying methyltransferase activity. This assay monitors the presence of SAH, and can be used for high-throughput screening.

Suggested uses

  • Disease assays
  • Drug development
  • Chemical processing

Advantages

  • Sensitive and selective means to assay methyltransferase activity
  • Fluorescent marker compatible with current imaging systems
  • High-throughput screening can be used to identify new methyltransferases and their activity parameters
  • Identification of substrates and inhibitors

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Inventors

  • Hammond, Ming Chen

Other Information

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