New Collision-Induced Dissociation Cross Linker and Related Software Package for Fast and Accurate Mass Spectrometry Analysis of Proteins

Tech ID: 21327 / UC Case 2010-689-0

Brief Description

Researchers at the University of California, Irvine have developed a new and novel mass spectrometry (MS) cleavable disuccinimidyl sulfoxide (DSSO) cross-linker for MS analysis of proteins. This DSSO cross-linker contains two symmetric collision-induced dissociation (CID) cleavable sites that allow for effective identification of DSSO-cross-linked peptides based on their distinct fragmentation patterns unique to cross-linking types such as interlink, intralink, and dead-ends.

A software package was also developed to be used with the DSSO cross linker. This software performs an integrated data analysis workflow for identifying DSSO-cross-linked peptides.


This new cross-linker allows for the MS analysis of multi-subunit protein complexes. In addition, the accompanying software package allows for the fast and accurate identification of DSSO-cross-linked peptides via MS.

State Of Development

This cross-linker and its accompanying software have been successfully used for the structural characterization of yeast 20 S proteasome complex and also demonstrated is identifying and characterizing model peptides and proteins.

Patent Status

Country Type Number Dated Case
United States Of America Issued Patent 9,222,943 12/29/2015 2010-689
United States Of America Published Application 20170082635 03/23/2017 2010-689


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  • Huang, Lan
  • Rychnovsky, Scott D.

Other Information


mass spec, mass spectrometry, MS, cross-linkers, CID

Categorized As

  • Institute for Innovation Logo
  • Institute for Innovation Logo

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