METHOD FOR EXTENSION OF READ LENGTHS FOR ULTRA HIGH-THROUGHPUT SEQUENCING
Tech ID: 18992 / UC Case 2009-009-0
Brief Descripton
BACKGROUND:Several commonly used commercial ultra high-throughput (HTP) sequencing technologies share a common limitation: the instruments produce relatively short reads, typically shorter than 40 bases long. If longer reads are desirable, additional cycles can be run, but these additional cycles result in a dramatically increased error rate. Some competing HTP sequencing technologies provide longer average read lengths of approximately 200 bases, but severely limit the number of sequences that can be produced, namely 1/100 to even 1/1000 the number of sequences that the shorter read-length technologies can produce at equivalent overall cost. Thus, there is a need for new methods that would allow for longer read lengths for technologies able to produce in the tens or hundreds of millions of sequences. DESCRIPTION: UCSF scientists have developed a new method for significantly extending the read lengths obtained from ultra HTP sequencing technologies. This technique leverages the depth and redundancy of sequencing obtained from the ultra HTP sequencing technologies that currently produce tens or hundreds of millions of sequences to result in increased read lengths of up to approximately 130 bases. This method has been validated on both the Saccharomyces cerevisiae transcriptome and the more challenging Plasmodium falciparum transcriptome using an ultra high sequence number instrument.
Features/Benefits
- 2.5-3 times the currently achievable read length of the ultra HTP sequencing technologies
Applications
- Whole genome sequencing
- Functional genomics
- SNP detection
- Gene and/or pathogen discovery
- Metagenomics
Other Information
Categorized As
Related cases
2009-009-0
Keywords
sequencing
Contact
Karin H. Immergluck / karin.immergluck@ucsf.edu / tel: View Phone Number. Please reference Tech ID #18992.
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