UCLA researchers in the Department of Mechanical and Aerospace Engineering have developed a tunable, sheathless, and three dimensional single-stream cell focusing in high speed flows. This new mechanism can be used for real-time focusing in flow cytometers and high-throughput cell sorting.
Cells and microparticles ﬂow at different speeds in different streamlines in microﬂuidic channels. The ability to three-dimensionally focus randomly distributed cells and microparticles into a single-stream is a critical function needed in numerous microﬂuidic applications such as ﬂow cytometry, cell sorting, and imaging.
The inventors have reported a novel dielectrophoretic (DEP) mechanism for tunable, sheathless, three-dimensional, and single-stream microparticle and cell focusing in high-speed flows. It is realized by fabricating a 3D microfluidic device with two glass substrates sandwiching a thin and open PDMS channel. Electrodes are laid out to provide DEP forces completely perpendicular to hydrodynamic flows along the entire channel. This new approach provides, for the first time, real-time 3D tuning of focusing locations by simply changing voltage combinations applied to the electrodes.
|United States Of America||Published Application||20170291172||10/12/2017||2016-485|