Researchers at the UCLA Department of Medical and Molecular Pharmacology have developed a compact microfluidic device that is able to achieve rapid concentration and/or reformulation of PET tracers after HPLC purification.
Positron emission tomography (PET) uses small amounts of short-lived radiolabeled tracers to image specific molecular processes in living subjects for clinical applications including disease diagnosis and disease progression monitoring. Synthesis of most PET tracers requires purification via semi-preparative high performance liquid chromatography (HPLC), and downstream solid-phase extraction (SPE) and/or evaporation to remove solvents while concentrating PET tracers for final formulation. Commercially available evaporation systems (i.e. rotary evaporators) used for solvent evaporation are extremely bulky and require manual intervention to assess the vacuum level, introduce saline, monitor the evaporation process, and collect the formulated final product. Compact vortex evaporator systems and microfluidic chip architectures have been reported for solvent evaporation but are not suitable for PET tracer formulation since their evaporation rates are too low for time-sensitive concentration of short-lived radioactive tracers.
Researchers at UCLA have developed a compact microfluidic device architecture that is able to achieve complete evaporation of solvent during the PET tracer concentration process at a fast speed to minimize radioactive decay of the PET tracer. This microfluidic system has been designed to minimize dead volume, facilitate heat flow throughout the system to improve evaporation rate, and completely automate the evaporation process. An additional solvent exchange step is also enabled in this system to thoroughly remove organic solvents and ensure that the final concentrated formulation is safe for injection.
Prototype has been developed.
|European Patent Office||Published Application||EP 3510393||07/17/2019||2017-185|
|United States Of America||Published Application||20190201560||07/04/2019||2017-185|
Positron emission tomography, PET, PET tracer, microfluidic, solvent removal, evaporation, formulation, reformulation