Massively Parallel High Throughput Single Cell Electroporation (MSEP)

Tech ID: 30166 / UC Case 2017-086-0

Summary

UCLA researchers in the Department of Mechanical and Aerospace Engineering have developed a novel massively parallel, single cell electroporation platform (MSEP) that is high throughput, efficient, and maintains cell viability.

Background

Electroporation is clinically used to deliver various extracellular molecules (i.e. drugs, proteins, nucleic acids, nanoparticles, etc.) to cells, tissues, and organisms. However, conventional bulk electroporation techniques require high voltage sources in order to electroporate cells and deliver cargo, resulting in a high percentage of cell death. Microfluidic electroporation devices can provide high delivery efficiency and high cell viability through better-controlled electric fields, but their throughput is orders of magnitude lower than bulk approaches.

Innovation

Professor Chiou and his research team have developed a novel MSEP that not only overcomes the throughput limitations of microfluidic-based approaches but also uses low voltage sources for high efficiency electroporation with high cell viability post-delivery. This compact, easy-to-use silicon device can deliver cargo in up to 10 million cells/min on a 1 cm2 chip, where calcium dyes, large-sized dextran proteins, and plasmids were successfully delivered to mammalian cells with high efficiency (up to 90%) and high cell viability (up to 90%).

Applications

  • Cargo delivery via electroporation 
  • Drug delivery 
  • Gene editing material delivery

Advantages

  • Delivery of a diverse range of cargo (drugs, proteins, nucleic acids, nanoparticles) 
  • Compact and easy to use 
  • Low voltage 
  • Ultrahigh throughput 
  • High delivery efficiency and high cell viability

State Of Development

Prototype microfluidic devices have been developed and extensively tested. Cargo delivery (calcium dyes, large-sized dextran proteins, and plasmids) to mammalian cells were successfully demonstrated with high throughput, efficiency, and minimal cell death.

Patent Status

Country Type Number Dated Case
United States Of America Published Application 20180066222 03/08/2018 2017-086
 

Contact

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Inventors

  • Chiou, Pei Yu E.

Other Information

Keywords

Electroporation, microfluidics, massively parallel single-cell electroporation, MSEP, ultrahigh throughput, drug delivery, cargo delivery, cell delivery, gene delivery

Categorized As