Researchers at the University of California, Davis have developed a highly sensitive immunosensor for the detection of chloramphenicol (CAP) residues in milk and other protein-based substances.
Current technologies for the detection of antibiotics in proteins include poly(vinyl alcohol-co-ethylene) (PVA-co-PE)-casted membranes. However, these immunosensors are not sensitive enough to detect small amounts of antibiotics. They also require treatment of the proteins to label them. Therefore, there exists a need for a more efficient immunosensor.
Researchers at the University of California, Davis have developed an ultra-sensitive, amperometric immunosensor for the detection of CAP. No pre-treatments are required for testing for residues in milk. This immuno-sensor utilizes a nano-fibrous membrane, which decreases the electron transfer resistance significantly compared with the standard PVA-co-PE-casted membrane.
The antibiotics of CAP provided high selectivity and sensitivity to CAP, while nano-fibrous membrane structures provide ultra-high surface areas and porosity. This design makes the sensor highly sensitive and selective, easy to use and highly responsive.
This immunosensor shows a high sensitivity to even low levels of target antibiotics in label-free milk samples or other proteins. It demonstrates good selectivity, reusability and stability over time. The sensor’s structural platform design also shows promise for fabricating other types of chemical sensors.