Label-Free, Electrochemical Sensor for Detection of Antibiotics in Protein-based Substances

Tech ID: 30098 / UC Case 2018-633-0

Abstract

Researchers at the University of California, Davis have developed a highly sensitive immunosensor for the detection of chloramphenicol (CAP) residues in milk and other protein-based substances.

Full Description

Current technologies for the detection of antibiotics in proteins include poly(vinyl alcohol-co-ethylene) (PVA-co-PE)-casted membranes. However, these immunosensors are not sensitive enough to detect small amounts of antibiotics. They also require treatment of the proteins to label them. Therefore, there exists a need for a more efficient immunosensor.

Researchers at the University of California, Davis have developed an ultra-sensitive, amperometric immunosensor for the detection of CAP. No pre-treatments are required for testing for residues in milk. This immuno-sensor utilizes a nano-fibrous membrane, which decreases the electron transfer resistance significantly compared with the standard PVA-co-PE-casted membrane.

The antibiotics of CAP provided high selectivity and sensitivity to CAP, while nano-fibrous membrane structures provide ultra-high surface areas and porosity. This design makes the sensor highly sensitive and selective, easy to use and highly responsive.

This immunosensor shows a high sensitivity to even low levels of target antibiotics in label-free milk samples or other proteins. It demonstrates good selectivity, reusability and stability over time. The sensor’s structural platform design also shows promise for fabricating other types of chemical sensors.

Applications

  • Label-free sensor for CAP antibiotics in milk proteins

Features/Benefits

  • System can be used to fabricate other antibiotic or chemical sensors
  • Does not require pretreatment of the milk or other proteins
  • Ultrahigh sensitivity to detect low levels of antibiotics

Patent Status

Patent Pending

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Inventors

  • El-Moghazy, Ahmed
  • Si, Yang
  • Sun, Gang
  • Zhao, Cunyi

Other Information

Keywords

Biosensors, Bioelectronics, Amperometry, Immunosensors

Categorized As