Researchers at the University of California, Davis have developed a guided nuclease based expression system to introduce genetic modifications into plants without the need for tissue culture.
Plant breeding methods rely largely on existing genetic variants as well as random mutagenesis, for the improvement of crops. With the development of genome editing technologies, it is possible to identify, clone and modify specific genes to influence desired traits such as mass yield, plant architecture, stress resistance and nutritional value. These methods, however, currently require tissue culture, which involves transforming the designed constructs into cells, selecting for transgene integration, and generation of parent and daughter plants to grow and confirm modification from the constructed cells. These processes can be expensive, time-consuming, and are inaccessible to small research labs.
Researchers at the University of California, Davis have developed a transient expression system that can deliver multiple guide RNAs to plants expressing guided nucleases such as Cas9. The method generates edited plants without the need for stable integration of transgenes or regeneration through tissue culture. This technology produces CRISPR-edited plants without the costs of tissue culture based approaches and, moreover, these mutations are heritable.
guided nuclease, sgRNA, CRISPR, Cas9, CRISPR-Cas9, mutagenesis, tissue culture, plant, gene editing, heritable