Researchers at the University of California Davis have developed a thaw gelation process for the formation of cell spheroids within a hydrogel shell.
Cell spheroids allow for the interaction of cells both with each other and the extracellular environment. These spheroids can be encapsulated in shells, creating a shell and core structure, to deliver or test the effect of cargo, such as small molecules and drugs, on cellular responses in a 3D environment. These 3D structures, however, are limited in their ability to form hydrogel shells around cell cultures and may require specialized equipment for making organoids.
Researchers at the University of California Davis have developed a thaw gelation process that enables the formation of cell spheroids within a hydrogel shell. Briefly, cells are frozen in a special freezing solution and, when poured into a special thawing solution, the solutions rapidly gel and encapsulate the cells in a hydrogel shell. The shell retains the shape of the molded disc and can act as a reservoir for the compartmentalization and isolation of distinct cell populations. The shell can also be degraded via enzyme for the controlled release of encapsulated contents. This method does not require specialized wells or drop holding equipment and reduces the spheroid formation time as cells can be encapsulated straight out of cryopreservation.
spheroids, cryopreservation, encapsulation, sacrificial, gelation, hydrogel shell