UCLA researchers in the Department of Bioengineering have developed a method to form a biologically functional lipid bilayer in a high-throughput and automated fashion.
Currently medium throughput ion channel measurements are conducted using automated patch-clamping. This technique involves directly attaching to a cell and measuring the current through a specific ion channel embedded in the cell membrane. The process of automated patch-clamping is a relatively slow and costly technique. Artificial bilayers, which are created in a laboratory for ion channel insertion and study, are attractive since the measurement is much easier and the experimenter has greater control over the surrounding biological environment. Yet, as a technology, artificial lipid bilayers are also characterized by low throughput and relatively high costs associated with its manual technique.
Researchers at UCLA have developed a method to form a biologically functional lipid bilayer in a high- throughput and automated fashion. The ionic current that flows through the channel proteins incorporated into the bilayer can be directly monitored.
This technology has been successfully demonstrated in the laboratory.
|United States Of America||Issued Patent||9,068,280||06/30/2015||2010-054|
Lipid bilayer, lipid, bilayer, sessile droplets, sessile, droplets, high-throughput, automated, ionic current, protein channels, artificial bilayer, protein incorporation, protein measurement