Method to Develop a Stable Pluripotent Bovine Embryonic Stem Cell Line

Tech ID: 29528 / UC Case 2018-056-0

Abstract

Researchers at the University of California, Davis have developed a method to produce stable pluripotent bovine embryonic stem cells.

Full Description

Cattle are one of the most important domestic ungulates commonly used for food and bioreactors. Development of a domestic ungulate embryonic stem cell line (ESCs) is important for genomic testing and selection, genetic engineering, and providing an experimental tool for studying human diseases. To date, however, it has remained a challenge to produce stable pluripotent bovine ESCs.

Researchers at the University of California, Davis have developed a method to produce a pluripotent bovine ESC line (bESC). The culture system produces bESCs with stable morphology, karyotype, pluripotency marker expressions, and epigenetic profiles. The derived line has high efficiency, is simple to propagate and becomes established in 3 to 4 weeks. This patent pending method can also be used to create cells as donors for nuclear transfer by utilizing bESCs produced with normal blastocysts rates, opening the possibility to use them for genomic selection, genome editing, and production of cattle with high-genetic value.

Applications

  • Production of stable pluripotent bovine ESCs
  • Genomic testing and selection
  • Genetic engineering
  • Experimental tool for studying human diseases

Features/Benefits

  • Produces bESCs with stable morphology, karyotype, pluripotency marker expressions, and epigenetic profiles
  • Can be used as donors for nuclear transfer
  • High efficiency
  • Cell line easy to propagate
  • Established line in 3 to 4 weeks

Patent Status

Patent Pending

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Inventors

  • Bogliotti, Yanina
  • Ross, Pablo
  • Vilarino, Marcela

Other Information

Keywords

ungulate, pluripotent bovine embryonic stem cells, bovine stem cells, bESC, bovine ESC, culture system, genomic testing, genetic engineering

Categorized As