Protein-Coated Microparticles For Protein Standardization In Single-Cell Assays

Tech ID: 29186 / UC Case 2018-103-0

Patent Status

Country Type Number Dated Case
United States Of America Published Application 20190242883 08/08/2019 2018-103
 

Additional Patent Pending

Brief Description


Single-cell analysis offers powerful capabilities of identification of rare sub-populations of cells, understanding heterogeneity of cancerous tumors, and tracking cell differentiation and reprogramming. Despite great potentials for uncovering new biological systems and targeting diseases with precision medicine, single-cell approaches are composed of complex device processes that can cause bias in measurement.  In deep sequencing, technical variation in single cell expression data occurs during capture and pre-amplification steps. Similarly, in single-cell protein assays, technical variability can obscure functionally relevant variance. 

 

To better control protein measurement quality in single-cell assays, researchers at the University of California, Berkeley developed a novel method to loading and release protein standard. This method utilizes the surface of modified and functionalized microparticles as vehicles to capture target proteins with desired concentrations. Chelation-assisted click chemistry is applied to demonstrate that protein standards with different molecular masses can be loaded and bounded in a single-cell protein assay. Microparticles are introduced into single-cell devices by either passive gravity, magnetic attraction, or other physicochemical forces. These protein standards from microparticles provide a reference to measure protein mass sizes from individual cells and a quality control for any biases in device fabrication, cell lysis, protein solubility, protein capture, and protein readouts (i.e. antibody probing). 

 

Suggested uses


  • Standards for assessing technical variation in microfluidic devices (e.g. single-cell electrophoretic cytometry)

 

Advantages


  • Proteins or biomaterials can be released and measured optically in situ

 

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Inventors

  • Herr, Amy E.

Other Information

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