Small RNA Extraction Kit with High Yield

Tech ID: 29078 / UC Case 2018-130-0

Background

Small RNAs are noncoding RNAs shorter than 100 nucleotides. One of the most commonly studied small RNA families are microRNAs (miRNA) that can bind to target mRNAs and inhibit their translation or induce degradation. A functional study of miRNAs and exploration of their utility as disease markers require miRNA extraction from biological samples, which contain large amounts of interfering compounds for downstream RNA identification and quantification. The most common extraction methods employ silica columns or the TRIzol reagent but these result in low levels of small RNA recovery.

Brief Description

Prof. Wenwan Zhong and her lab at UCR have developed a method to recover small RNAs using TiO2 fibers which results in a 200-fold improvement in yield when compared to commercially available SiO2 columns.

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Fig. 1 Schematic of the steps involved in recovering small RNAs using TiO2 fibers and a unique protocol to wash and elute the small RNA.

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Fig. 2 Extracting miRNA from MDA-MB-231 cells with TiO2 fibers and SiO2 PureLink miRNA isolation columns. Higher recoveries of endogenous hsa-miR-21 were found with TiO2 fibers when compared with PureLink columns.

Applications

  • Efficient and robust extraction of small RNAs for diagnostic and research purposes.

Related Materials

Patent Status

Patent Pending

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Keywords

electrospun, miRNA, TiO2, titanium dioxide, extraction, RNA

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