High-Efficiency One-Cell-One-Bead Encapsulation In Droplets

Tech ID: 28769 / UC Case 2017-803-0

Brief Description

A high-efficiency single-cell droplet encapsulation method to improve single cell pharmacological assay throughput.

Full Description

Background:

  • Cell and bead droplet encapsulation is common technique used in heterogeneous single cell pharmacology assays probing genomics, and proteomics.
  • Current solutions for one bead-one cell encapsulation are based on random encapsulation dictated by Poisson statistics.

Problem:

  • Low (0.1%) efficiency of one bead-one cell droplet encapsulation limits droplet sequencing and heterogeneous assays throughput

Solution:

  • Increased one bead-one cell encapsulation efficiency (> 300 times current techniques) utilizing a controllable hydrodynamic vortices microfluidic platform

Suggested uses

  • Increased throughput of genome-wide expression profiles for individuals cells
  • High efficiency one-bead-one cell encapsulation for droplet based heterogeneous single cell assays

Advantages

  • Improved cell-bead encapsulation throughput for droplet sequencing applications of single cell genomics and proteomics
  • Improving the encapsulation efficiency of droplet containing a single cell and a single bead compared to encapsulation of either component individually.

Patent Status

Patent Pending

Contact

Learn About UC TechAlerts - Save Searches and receive new technology matches

Other Information

Categorized As

  • AN INTEGRAL PART OF
  • Institute for Innovation Logo
  • Institute for Innovation Logo

University of California, Irvine Invention Transfer Group
5141 California Avenue, Suite 200, Irvine,CA 92697-7700
Tel: 949.824.2683 | Fax: 949.824.3880
www.ota.uci.edu | ota@uci.edu

© 2017, The Regents of the University of California
Terms of use | Privacy Notice