Professors in the UCLA Department of Bioengineering have developed a novel short oligonucleotide assay to fluorescently detect biomolecules.
The ability to quickly and accurately detect biomolecules indicative of disease is crucial given importance of early diagnosis in disease treatment. The current gold-standard in the field of biomolecule detection is the enzyme-linked immunosorbent assay or ELISA, however, current ELISA-based diagnostic assays suffer from various limitations. ELISA assays require a significant number of sample preparation and wash steps which are a hindrance to their use in point-of-care (POC) diagnostic settings; their use in clinical microbiology settings or high throughput screens requires significant automation and remains robotics-intensive. Additionally, immunoassays currently available for the detection of specific analytes suffer from sensitivity issues as they lack signal amplification. Therefore, there remains a need for novel diagnostic assays with increased sensitivity, decreased overall sample preparation and handling, and greater usability for POC diagnostic settings.
Professor Di Carlo and colleagues have developed a novel biomolecule detection assay utilizing small oligonucleotides which immediately generates signal in the presence of a specific analyte. HEBA is a single-pot assay capable of detecting the presence of a biomolecule in relevant bio fluids at room temperature in 10 minutes. HEBA overcomes multiple limitations of current molecule detection assays as it does not require washing steps or temperature cycling, is enzyme independent, does not require immobilization at a surface, and is sensitive to the attomolar range in a microfluidic digital assay platform. Compared to current assay methods HEBA improves the level of detection of analyte, is performed as a homogeneous assay and requires only ten minutes for detection.
To date the technology has demonstrated efficacy in detecting influenza biomolecules in relevant buffers, whole blood and undiluted plasma.
|United States Of America||Published Application||20180179576||06/28/2018||2015-579|
biomolecular assay, oligonucleotide sequence assay, analyte recognition, immunoassay, molecular detection, diagnostic assay, fluorescent, fluorescence